All environmental and subject preconditioning protocols recommended for Laser Doppler Perfusion measurements should be considered. Capillary blood flow is influenced by many factors so it is best to control as many known influences as possible. These include:
using a temperature controlled room,
acclimatising the subject for a set period before any measurements are taken,
prohibiting the intake of coffee or smoking before or during a measurement,
reducing stress,
using a consistent position: supine, sitting or standing, To reduce movement artifacts it is best to have the subject laying in a supine position.